Peripheral Blood Stem Cell Collection

Estimate CD34+ yield using pre-apheresis peripheral blood CD34+, liters processed, collection efficiency, and recipient weight. For educational use only; clinical decisions require validated laboratory assays and apheresis protocols.

Peripheral blood stem cell collection (predicted CD34+ yield)

Enter pre-apheresis peripheral CD34+ (×10⁶ cells/L), blood volume processed (L), collection efficiency (%), and recipient weight (kg). The tool estimates total CD34+ cells collected and dose per kg. For teaching and rough checks only.

Recipient weight (kg)

Processed blood (L)

Peripheral CD34+ (×10⁶ cells/L)

Use the absolute CD34+ count from your lab in ×10⁶ cells/L (same unit as many flow reports).

Collection efficiency (%)

Fraction of circulating CD34+ cells captured into the product, expressed as a percent. Use your program or device-specific estimate.

Disclaimer: Educational estimate only. PBSC collection requires validated CD34+ assays, specialized equipment, and transplant or apheresis team oversight. This tool does not replace institutional protocols or laboratory QC.

What this estimates

During peripheral blood stem cell collection (apheresis), the predicted number of CD34+ cells collected depends on how many CD34+ cells were circulating before the run, how much blood volume was processed, and how efficiently the device captured those cells into the product.

This page uses the usual multiplicative yield model aligned with common transplant planning tools. Always use laboratory CD34+ results from validated instruments and your apheresis center's efficiency assumptions.

Symbols

C: peripheral blood CD34+ concentration, in ×10⁶ cells/L
V: total processed blood volume in liters
E: collection efficiency as a percent (0–100), reflecting what fraction of circulating CD34+ cells ends up in the collection product for that procedure
W: recipient weight in kg (used to express dose per kg)

Predicted total CD34+ yield

Total predicted CD34+ cells in the collection bag, expressed in ×10⁶ cells (millions of cells):

Total (×10⁶ CD34+ cells) = C × V × (E ÷ 100)

CD34+ dose per kg

Stem cell dose is often reported as ×10⁶ CD34+ cells per kg of recipient body weight:

Dose (×10⁶ cells/kg) = Total (×10⁶ cells) ÷ W

Limitations

• Efficiency varies by device, operator, interface settings, diagnosis, and whether the run is steady state or declining CD34+ counts.
• Pre-apheresis CD34+ may not reflect intra-procedure mobilization dynamics; multiple days or mobilization agents change planning.
• Clinical decisions must follow validated laboratory assays and your transplant or apheresis program protocols, not this estimate alone.

CalcMD.

Peripheral Blood Stem Cell Collection

Estimate CD34+ yield using pre-apheresis peripheral blood CD34+, liters processed, collection efficiency, and recipient weight. For educational use only; clinical decisions require validated laboratory assays and apheresis protocols.